Avidin



Avidin is a protein purification. Because of its carbohydrate content and basic pI, avidin has relatively high nonspecific binding.

Relationship between avidin and biotin

Avidin has a very strong affinity for biotin with a KD (streptavidin or NeutrAvidin, which also bind very tightly to Biotin, are often used in place of avidin.

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including western blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.

References

  1. ^  Bayer, Ed: "The Avidin-Biotin Complex", Dept. of Biological Chemistry, Weizmann Institute of Science, Israel
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Ricin
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Vitronectin
 
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