Cycloheximide



Cycloheximide
IUPAC name 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl
-2-oxocyclohexyl]-2-hydroxyethyl}
piperidine-2,6-dione
Other names naramycin a, hizarocin
actidione®, actispray
kaken, U-4527
Identifiers
CAS number 66-81-9
RTECS number MA4375000
SMILES C[C@H]1C[C@H](C)C(=O)[C@@H]
(C1)[C@@H](O)CC2CC(=O)NC(=O)C2
Properties
Molecular formula C15H23NO4
Molar mass 281.35
Appearance colourless crystals
Melting point

119.5-121°C

Hazards
EU classification T+, N
Main hazards Highly toxic
NFPA 704
 
4
4
 
S-phrases S45
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

Cycloheximide is an translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.

Due to significant toxic side effects, including antibiotic compound. Although it has been used as a fungicide in agricultural applications, this application is now decreasing as the health risks have become better understood.

Cycloheximide is degraded by alkali (pH > 7), decontamination of work surfaces and containers can be achieved by washing with a non-harmful alkali solution such as soap.

In vitro applications

Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a Western blotting of the cell lysates for the protein of interest can show differences in protein expression due to interference with translation.

References

  1. ^ [1]
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Cycloheximide". A list of authors is available in Wikipedia.