Deoxyribozyme



Deoxyribozymes or DNA enzymes or catalytic DNA, or DNAzymes are monomers both RNA and DNA have a much more restricted set of monomers (4) to choose from which limits the construction of interesting catalytic sites. For these reasons DNAzymes exist only in the laboratory.

Discovery

The first deoxyribozyme was discovered in 1994 [1] by current Yale Professor Ronald R. Breaker while a postdoctoral fellow in the laboratory of Prof. organic chemistry. DNAzymes have found practical use in metal biosensors[3].

This link and this link describes the DNA molecule 5'-GGAGAACGCGAGGCAAGGCTGGGAGAAATGTGGATCACGATT-3' which acts as a deoxyribozyme that uses light to repair a thymine dimer, using serotonin as cofactor.

Usage

With the aid of Solid-phase synthesis.

enantiomeric excess of 50%.

Other uses of DNA in chemistry are in DNA-templated synthesis, DNA nanowires and DNA computing[5].

See also

References

  1. ^ Breaker RR,; Joyce GF. (Dec 1994). "A DNA enzyme that cleaves RNA.". Chem Biol. 1 (4): 223-9. doi:10.1016/1074-5521(94)90014-0. PMID 9383394.
  2. ^ Scott K. Silverman (2004). "Deoxyribozymes: DNA catalysts for bioorganic chemistry". Org. Biomol. Chem. 2: 2701-06. doi:10.1039/B411910J.
  3. ^ Juewen Liu; Yi Lu (2004). "Optimization of a Pb2+-Directed Gold Nanoparticle/DNAzyme Assembly and Its Application as a Colorimetric Biosensor for Pb2+". Chem. Mater. 16 (17): 3231-38. doi:10.1021/cm049453j.
  4. ^ Gerard Roelfes; Ben L. Feringa (2005). "DNA-Based Asymmetric Catalysis". Angewandte Chemie International Edition: 3230-2. doi:10.1002/anie.200500298.
  5. ^ Yoshihiro Ito; Eiichiro Fukusaki (2004). "DNA as a ‘Nanomaterial’". Journal of Molecular Catalysis B: Enzymatic 28: 155–166.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Deoxyribozyme". A list of authors is available in Wikipedia.