Fast protein liquid chromatography

Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of affinity chromatography).

Trademark

FPLC is a trademark of GE Healthcare, formerly Amersham-Pharmacia Biotech and Pharmacia Biotech.

Typical columns

Typical columns used for protein purification include:

• Ion exchange chromatography, cation or anion — separate proteins based on surface-charges
• Size exclusion chromatography, also known as Gel filtration chromatography, Gel permeation chromatography or desalting — separate proteins based on size.
• Reversed phase or hydrophobic interaction — separates based on hydrophobicity which depends heavily on surface area
• Affinity chromatography — columns which purify based on ligand affinity (e.g. a His₆-tagged protein purified on a nickel column).

See also

• Chromatography, an overview article covering all chromatographic techniques.
• High performance liquid chromatography (HPLC) for column chromatography using high pressure.
• Affinity chromatography