In situ hybridization

In situ hybridization (ISH) is a type of Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (hybridization histochemistry) is used to measure and localize mRNAs and other transcripts within tissue sections or whole mounts.

Process

For hybridization histochemistry, sample cells and tissues are usually treated to fix the target transcripts in place and to increase access of the probe. As noted above, the probe is either a labeled immunohistochemistry, respectively. ISH can also use two or more probes, labeled with radioactivity or the other non-radioactive labels, to simultaneously detect two or more transcripts.

References

1. Jin L, Lloyd RV. In situ hybridization: methods and applications. J Clin Lab Anal. 11(1):2-9, 1997. PMID 9021518
2. Comprehensive and annotated in situ hybridization histochemistry