Micrococcal nuclease



Micrococcal Nuclease (S7 RNA and all sequences will be ultimately cleaved.

Characteristics

The molecular weight of 16.9kDa.

The EDTA.

Source

This enzyme is the extracellular nuclease of Staphylococcus aureus. Two strains, V8 and Foggi, yield almost identical enzymes.[2] A common source is E.coli cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease).

Applications

  • Hydrolysis of nucleic acids in crude cell-free extracts.
  • Sequencing of RNA.
  • Preparation of rabbit reticulocytes lysates
  • Studies of chromatin structure.
  • Remove nucleic acids from protein preparation allowing for folding and structure-function studies.

References

  1. ^ Heins JN, Suriano JR, Taniuchi H, Anfinsen CB (1967). "Characterization of a nuclease produced by Staphylococcus aureus". J. Biol. Chem. 242 (5): 1016-20. PMID 6020427.
  2. ^ Cusumano CL, Taniuchi H, Anfinsen CB (1968). "Staphylococcal nuclease (Foggi strain). I. Order of cyanogen bromide fragments and a "fourth" histidine residue". J. Biol. Chem. 243 (18): 4769-77. PMID 5687719.
  • http://www.fermentas.com/catalog/modifyingenzymes/nucleasemicrococcal.htm
  • http://www.worthington-biochem.com/NFCP/default.html
  • http://www.fermentas.com/pdf/msds/en0181_eu.pdf#search=%22micrococcal%20nuclease%22 - A material and safety data sheet for the product
v • d • e
RNA-induced silencing complex)
either deoxy- or ribo- (Aspergillus nuclease S1, Micrococcal nuclease)
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Micrococcal_nuclease". A list of authors is available in Wikipedia.